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Submicron‐Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology

Zhang, Wenbo ; Bertinetti, Luca ; Yavuzsoy, Efe Cuma ; Gao, Changyou ; Schneck, Emanuel ; Fratzl, Peter (2023)
Submicron‐Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology.
In: Advanced Healthcare Materials, 2023, 12 (9)
doi: 10.26083/tuprints-00023694
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Item Type: Article
Type of entry: Secondary publication
Title: Submicron‐Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology
Language: English
Date: 26 May 2023
Place of Publication: Darmstadt
Year of primary publication: 2023
Publisher: Wiley-VCH
Journal or Publication Title: Advanced Healthcare Materials
Volume of the journal: 12
Issue Number: 9
Collation: 11 Seiten
DOI: 10.26083/tuprints-00023694
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

Physical forces are important cues in determining the development and the normal function of biological tissues. While forces generated by molecular motors have been widely studied, forces resulting from osmotic gradients have been less considered in this context. A possible reason is the lack of direct in situ measurement methods that can be applied to cell and organ culture systems. Herein, novel kinds of resonance energy transfer (FRET)‐based liposomal sensors are developed, so that their sensing range and sensitivity can be adjusted to satisfy physiological osmotic conditions. Several types of sensors are prepared, either based on polyethylene glycol‐ (PEG)ylated liposomes with steric stabilization and stealth property or on crosslinked liposomes capable of enduring relatively harsh environments for liposomes (e.g., in the presence of biosurfactants). The sensors are demonstrated to be effective in the measurement of osmotic pressures in pre‐osteoblastic in vitro cell culture systems by means of FRET microscopy. This development paves the way toward the in situ sensing of osmotic pressures in biological culture systems.

Uncontrolled Keywords: biosensing, imaging, liposomes, resonance energy transfer, semi‐permeable membranes
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-236946
Classification DDC: 500 Science and mathematics > 530 Physics
500 Science and mathematics > 570 Life sciences, biology
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics > Soft Matter Biophysics
Date Deposited: 26 May 2023 12:06
Last Modified: 29 Sep 2023 07:42
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23694
PPN: 511959567
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