TU Darmstadt / ULB / TUprints

Codon Bias Can Determine Sorting of a Potassium Channel Protein

Engel, Anja J. ; Kithil, Marina ; Langhans, Markus ; Rauh, Oliver ; Cartolano, Matea ; Etten, James L. Van ; Moroni, Anna ; Thiel, Gerhard (2021):
Codon Bias Can Determine Sorting of a Potassium Channel Protein. (Publisher's Version)
In: Cells, 10 (5), MDPI, e-ISSN 2073-4409,
DOI: 10.26083/tuprints-00019352,
[Article]

[img]
Preview
Text
cells-10-01128 (1).pdf
Available under CC BY 4.0 International - Creative Commons, Attribution.

Download (2MB) | Preview
Item Type: Article
Origin: Secondary publication via sponsored Golden Open Access
Status: Publisher's Version
Title: Codon Bias Can Determine Sorting of a Potassium Channel Protein
Language: English
Abstract:

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.

Journal or Publication Title: Cells
Journal volume: 10
Number: 5
Publisher: MDPI
Collation: 17 Seiten
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 25 Aug 2021 12:25
Last Modified: 25 Aug 2021 12:25
DOI: 10.26083/tuprints-00019352
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-193522
Additional Information:

Keywords: codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting

URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19352
Export:
Actions (login required)
View Item View Item