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A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae

Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Van Etten, James L. ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver (2021):
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. (Publisher's Version)
In: Viruses, 12 (10), MDPI, ISSN 1999-4915,
DOI: 10.26083/tuprints-00019251,
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Item Type: Article
Origin: Secondary publication via sponsored Golden Open Access
Status: Publisher's Version
Title: A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae
Language: English
Abstract:

Potassium ion (K+) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K+ channels among these alga-infecting viruses has thus far been restricted to members of the family Phycodnaviridae, which are large, double-stranded DNA viruses within the phylum Nucleocytoviricota. Recent sequencing projects revealed that alga-infecting members of Mimiviridae, another family within this phylum, may also contain genes encoding K+ channels. Here we examine the structural features and the functional properties of putative K+ channels from four cultivated members of Mimiviridae. While all four proteins contain variations of the conserved selectivity filter sequence of K+ channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K+ channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K+ over Na+ and a sensitivity to Ba2+. Thus, virus-encoded K+ channels are not limited to Phycodnaviridae but also occur in the members of Mimiviridae. The large sequence diversity among the viral K+ channels implies multiple events of lateral gene transfer.

Journal or Publication Title: Viruses
Journal volume: 12
Number: 10
Publisher: MDPI
Collation: 14 Seiten
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 03 Aug 2021 07:28
Last Modified: 03 Aug 2021 07:28
DOI: 10.26083/tuprints-00019251
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-192518
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19251
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