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Sister chromatid exchanges occur in G₂-irradiated cells

Conrad, Sandro ; Künzel, Julia ; Löbrich, Markus (2021):
Sister chromatid exchanges occur in G₂-irradiated cells. (Publisher's Version)
In: Cell Cycle, 10 (2), pp. 222-228. Taylor and Francis Group, ISSN 1538-4101, e-ISSN 1551-4005,
DOI: 10.26083/tuprints-00019036,
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Item Type: Article
Origin: Secondary publication service
Status: Publisher's Version
Title: Sister chromatid exchanges occur in G₂-irradiated cells
Language: English
Abstract:

DNA double-strand breaks (DSBs) are arguably the most important lesions induced by ionizing radiation (IR) since unrepaired or mis-repaired DSBs can lead to chromosomal aberrations and cell death. The two major pathways to repair IR-induced DSBs are non-homologous end-joining (NHEJ) and homologous recombination (HR). Perhaps surprisingly, NHEJ represents the predominant pathway in the G1 and G2 phases of the cell cycle, but HR also contributes and repairs a subset of IR-induced DSBs in G2. Following S-phase-dependent genotoxins, HR events give rise to sister chromatid exchanges (SCEs), which can be detected cytogenetically in mitosis. Here, we describe that HR occurring in G2-irradiated cells also generates SCEs in ~50% of HR events. Since HR of IR-induced DSBs in G2 is a slow process, SCE formation in G2-irradiated cells requires several hours. During this time, irradiated S-phase cells can also reach mitosis, which has contributed to the widely held belief that SCEs form only during S phase. We describe procedures to measure SCEs exclusively in G2-irradiated cells and provide evidence that following IR cells do not need to progress through S phase in order to form SCEs.

Journal or Publication Title: Cell Cycle
Journal volume: 10
Number: 2
Publisher: Taylor and Francis Group
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology > Radiation Biology and DNA Repair
Date Deposited: 08 Sep 2021 12:09
Last Modified: 08 Sep 2021 12:10
DOI: 10.26083/tuprints-00019036
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-190369
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19036
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