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Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering

Schäfer, Benedikt and Emonts, Caroline and Glimpel, Nikola and Ruhl, Tim and Obrecht, Astrid S. and Jockenhoevel, Stefan and Gries, Thomas and Beier, Justus P. and Blaeser, Andreas (2021):
Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering. (Publisher's Version)
In: Materials, 13 (16), MDPI, ISSN 1996-1944,
DOI: 10.26083/tuprints-00017847,
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Item Type: Article
Origin: Secondary publication via sponsored Golden Open Access
Status: Publisher's Version
Title: Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering
Language: English
Abstract:

Mesenchymal stem cells (MSCs) possess huge potential for regenerative medicine. For tissue engineering approaches, scaffolds and hydrogels are routinely used as extracellular matrix (ECM) carriers. The present study investigated the feasibility of using textile-reinforced hydrogels with adjustable porosity and elasticity as a versatile platform for soft tissue engineering. A warp-knitted poly (ethylene terephthalate) (PET) scaffold was developed and characterized with respect to morphology, porosity, and mechanics. The textile carrier was infiltrated with hydrogels and cells resulting in a fiber-reinforced matrix with adjustable biological as well as mechanical cues. Finally, the potential of this platform technology for regenerative medicine was tested on the example of fat tissue engineering. MSCs were seeded on the construct and exposed to adipogenic differentiation medium. Cell invasion was detected by two-photon microscopy, proliferation was measured by the PrestoBlue assay. Successful adipogenesis was demonstrated using Oil Red O staining as well as measurement of secreted adipokines. In conclusion, the given microenvironment featured optimal mechanical as well as biological properties for proliferation and differentiation of MSCs. Besides fat tissue, the textile-reinforced hydrogel system with adjustable mechanics could be a promising platform for future fabrication of versatile soft tissues, such as cartilage, tendon, or muscle.

Journal or Publication Title: Materials
Journal volume: 13
Number: 16
Publisher: MDPI
Collation: 16 Seiten
Classification DDC: 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik
Divisions: 16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD)
Date Deposited: 23 Mar 2021 08:11
Last Modified: 23 Mar 2021 08:11
DOI: 10.26083/tuprints-00017847
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-178471
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/17847
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